Assessing cartilage regeneration in a minipig model: cutting-edge MR techniques challenge the gold standard
Author Block: K. L. Radke, B. Valentin, A. Müller-Lutz, M. Frenken; Düsseldorf/DE
Purpose: The purpose of this study was to examine the potential of differently composed MR sequences, including T1, T2, T2* and T1ρ, in assessing cartilage regeneration. These MR techniques were compared to dGEMRIC, providing valuable insight into non-invasive methods for monitoring and assessing cartilage repair after surgery.
Methods or Background: In orthopaedics, assessing articular cartilage regeneration safely and effectively remains a challenge, despite advancements in compositional MRI. We aimed to investigate the efficacy of various compositional MR sequences in quantitatively assessing cartilage and compare them to the gold standard, delayed gadolinium-enhanced MR imaging of cartilage (dGEMRIC).
In a preclinical minipig model, we induced standardised osteochondral defects in the proximal femur of 14 animals, divided into four groups: porcine collagen scaffolds with autologous adipose stromal cells (ASC), autologous bone marrow stromal cells (BMSC), unpopulated scaffolds (US), and an untreated defect group. After six months, we employed different MR composition methods, including dGEMRIC, T1, T2, T2*, and T1ρ, using a clinical 3T MR scanner.
Results or Findings: Results showed significant differences in the untreated defect group, with lower dGEMRIC values (404.86±64.2 ms, P=0.018) and higher T2 times (44.24±2.75 ms, P<0.001) in the defect region. Conversely, there were no significant differences in dGEMRIC values among the three treatment groups (ASC, BMSC, US), indicating successful cartilage reconstruction.
Conclusion: Notably, dGEMRIC proved effective for monitoring cartilage regeneration. Interestingly, T2 imaging emerged as a reliable alternative, offering non-contrast cartilage imaging for future in vivo studies exploring different treatment modalities' cartilage regeneration potential.
Limitations: These results have not yet been translated from animal to human.
Funding for this study: Funding was received from the University of Düsseldorf, Deutsche Arthrose-Hilfe.
Has your study been approved by an ethics committee? Yes
Ethics committee - additional information: The study was approved by the Ethics Committee of the University of Düsseldorf.